https://vital.seals.ac.za/vital/access/manager/Index en-us 5 https://vital.seals.ac.za/vital/access/manager/Repository/vital:10567 Wed 12 May 2021 23:39:24 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:4312 Wed 12 May 2021 23:20:08 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:5105 Wed 12 May 2021 23:09:10 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:5257 Wed 12 May 2021 23:09:07 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:4955 Wed 12 May 2021 22:52:22 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:10656 Wed 12 May 2021 22:42:47 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:5711 Wed 12 May 2021 22:33:44 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:5738 Wed 12 May 2021 22:20:44 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:5289 Wed 12 May 2021 20:58:43 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:5109 Wed 12 May 2021 20:48:53 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:10395 Wed 12 May 2021 20:37:40 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:10522 Wed 12 May 2021 20:22:03 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:5710 0.05 in all cases) although seasonal trends were marked, with highest values consistently recorded during the warmer summer months. Investigations into the community structure showed that the ichthyofaunal community within salt marsh was composed almost exclusively of juveniles of estuarine dependant (category II) species, mainly juvenile Mugilidae (<20mm SL) that comprised up to 83% of all fish sampled. Hierarchical cluster analysis and multidimensional scaling did not identify any distinct spatial patterns in the ichthyofaunal community within the salt marsh. The absence of any spatial patterns in the community structure could be related to the absence of any significant spatial patterns in the physico-chemical (temperature, salinity and dissolved oxygen concentrations) and biological (water column and microphytobenthic algal concentrations) variables within the salt marsh (P > 0.05 in all cases). Temporal shifts in the ichthyofaunal community structure within the salt marsh were, however, evident largely reflecting the breeding cycles of individual species within the sub-region. Within the adjacent eelgrass beds, total ichthyofaunal abundances and biomass ranged between 8.4 and 49.4 ind.10m⁻² and between 2.9 and 94.5 g.wwt.10m⁻², respectively. Once again there were no distinct spatial patterns in the abundance and biomass values evident although seasonal patterns were marked. In contrast to the salt marsh, within the in the eelgrass community, there were a large number of adult individuals recorded. Again category II species, the estuarine dependent species, were numerically and gravimetrically dominant. The dominance of category II species reflects the marine dominance of Kariega Estuary. The remaining estuarine utilisation categories did not contribute significantly to abundance or standing stock totals. Hierarchical cluster analysis showed that the salt marsh and eelgrass beds represented two distinct habitats within the Kariega Estuary. Within the salt marsh, the family Mugilidae were numerically dominant contributing 83% of the total catch. Within the eelgrass beds, the sparid, Rhabdosargus holubi and representatives of the family Gobidae contributed 36.3% and 33.9% respectively to the total catch. Estuaries with a wide range of microhabitats have been demonstrated to support a more diverse ichthyofaunal community. Shallow water habitats in general are important areas for juvenile fish within estuaries. Taylor’s salt marsh provides an alternative shallow water habitat, occupied by a distinct ichthyofaunal community composition, with increased food availability and decreased predation pressure, for a wide range of fish species.]]> Wed 12 May 2021 20:18:30 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:10566 Wed 12 May 2021 20:17:09 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:10326 Wed 12 May 2021 20:11:42 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:10324 Wed 12 May 2021 20:06:44 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:5302 Wed 12 May 2021 20:03:30 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:5569 Wed 12 May 2021 19:37:43 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:4887 Wed 12 May 2021 19:33:49 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:3812 Wed 12 May 2021 19:28:38 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:4854 Wed 12 May 2021 19:22:58 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:5823 Wed 12 May 2021 19:10:35 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:5459 Wed 12 May 2021 19:06:06 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:5413 Wed 12 May 2021 18:50:48 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:5465 Wed 12 May 2021 18:48:02 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:5421 Wed 12 May 2021 18:41:05 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:10327 Wed 12 May 2021 18:40:49 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:10702 Wed 12 May 2021 18:38:06 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:10540 Wed 12 May 2021 18:35:06 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:4656 Wed 12 May 2021 18:14:54 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:3980 Wed 12 May 2021 17:56:09 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:5790 0.05 in all cases). There was no evidence of enhanced biomass and abundance values at stations occupied in the immediate vicinity of the front. Total average carnivorous zooplankton abundance was dominated by chaetognaths (Eukrohnia hamata Möbius 1875, Sagitta gazellae Ritler-Záhony 1909 and S. zetesios Fowler 1905) and euphausiids (Nematoscelis megalops Sars 1883, Euphausia longirostris Hansen 1908 and E. spinifera Sars 1883), which contributed up to 86.58 ± 32.91% of the total counts. The total average biomass was dominated by euphausiids and amphipods (Themisto gaudichaudii Guérin-Méneville 1825, Phronima sedentaria Forsskål 1775 and Vibilia armata Bovallius 1887) which contributed up to 71.45 ± 34.85% of the total counts. In general the populations of both the euphausiids and amphipods were dominated by females while the chaetognaths were dominated by juveniles. Numerical analysis identified two major zooplankton groupings within the survey area which did not coincide with the water masses within the survey area. The SIMPER procedure of the PRIMER package indicated differences between the groups were mainly attributed to changes in the abundance of the numerically dominant species rather than the presence or absence of individual species. The absence of any significant spatial patterns in the distribution of the carnivorous zooplankton suggests that the STC did not act as a biogeographical barrier during the present study. The mean feeding rates of the chaetognaths E. hamata, S. gazellae and S. zetesios were 1.82 ± 0.85prey d-1, 3.63 ± 2.08prey d-1 and 2.18 ± 0.59prey d-1, respectively. These rates correspond to a combined predation impact equivalent to <5% of the mesozooplankton standing stock or <10% of the mesozooplankton secondary production. Mesozooplankton, comprising mainly copepods was the dominant prey in the guts of the three chaetognath species. Total predation impact of the euphausiids, chaetognaths and amphipods, estimated using published daily ration data, on the mesozooplankton standing stock and secondary production ranged from 0.01% to 1.53% and from 0.03% to 30.54%, respectively. Among the carnivorous zooplankton, chaetognaths were generally identified as the dominant predators of mesozooplankton. Low predation impact of selected carnivorous zooplankton suggested that these organisms contributed little to the vertical carbon flux within the region of investigation during the study.]]> Wed 12 May 2021 17:48:20 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:4749 Wed 12 May 2021 17:40:08 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:10319 Wed 12 May 2021 17:26:24 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:4598 Wed 12 May 2021 17:25:39 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:4650 Wed 12 May 2021 17:24:41 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:3773 Wed 12 May 2021 17:13:34 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:5318 Wed 12 May 2021 17:10:01 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:10374 Wed 12 May 2021 17:09:29 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:9693 Wed 12 May 2021 16:38:24 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:10448 Wed 12 May 2021 16:34:22 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:5486 Wed 12 May 2021 16:17:43 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:10450 Wed 12 May 2021 16:15:48 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:10634 Wed 12 May 2021 16:11:13 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:5263 20 orders and >35 families. The copepod Pseudodiaptomus hessei dominated (59 %) the zooplankton and occurred in similar densities to those observed in other South African estuaries. Larval fish and zooplankton varied across seasons, peaking simultaneously in summer although zooplankton showed additional density peaks during the closed phase of some estuaries. Both plankton components were more abundant in the oligohaline and mesohaline zones within the estuaries. Freshwater input, estuary type and the biogeography of the area influenced the composition and structure of larval fish and zooplankton assemblages in these estuaries. The findings suggest that the estuaries are functioning as successful breeding areas for the larvae of endemic estuary-resident fish species and that these estuaries have to be managed to ensure an adequate freshwater supply to maintain the biological integrity of the ecosystem, specially the maintenance of the highly productive River-Estuary Interface (REI) regions.]]> Wed 12 May 2021 15:56:36 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:4818 Wed 12 May 2021 15:52:50 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:10325 Tue 14 Feb 2017 03:36:29 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:5483 Thu 26 Aug 2021 14:37:41 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:10601 Thu 13 May 2021 12:05:21 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:3927 3 h), depolymerizing the pectin backbone while releasing the inulins from within the chicory roots. Analysis of various mixtures of FOS by mass spectrometry, HPLC and ¹H-NMR was undertaken. Results indicated that MS with electrospray ionization and ¹H-NMR are capable of providing relative quantitative data of the FOS present in the mixtures. The pharmaceutical effects of various sc-FOS (0.5%, v/v) and SCFA (0.3%, v/v) on certain bacterial enzymes (β-glucuronidase, urease and β-glucosidase) associated with the formation of carcinogens were also studied. These enzyme activities were not directly influenced by the sc-FOS, but were found to be remarkably decreased by SCFA, pointing toward the prebiotic effect of FOS in intestinal microflora modulation.]]> Thu 13 May 2021 11:50:24 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:5460 Thu 13 May 2021 09:13:17 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:10313 Thu 13 May 2021 08:44:42 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:4594 Thu 13 May 2021 08:18:22 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:4368 Thu 13 May 2021 07:39:04 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:4305 Thu 13 May 2021 07:09:37 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:10659 Thu 13 May 2021 07:06:10 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:5576 Thu 13 May 2021 06:55:34 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:10701 Thu 13 May 2021 06:53:39 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:5406 Thu 13 May 2021 06:49:38 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:5352 20°C). The aim of the study was to develop size and temperature specific diets for H midoe through optimisation of dietary protein, energy and lipid levels. Abalone were cultured under farm-like conditions in three partially recirculating temperature controlled systems at either 18, 22 or 24°C and fed formulated diets containing graded levels of protein (18,22 and 26 %) and energy (11.6, 13.5 and 16.2 MJ.kg·I ). Abalone were stocked into baskets at 5 % of available of surface area (n=36) and each diet (n=9) was fed to four baskets of abalone at each of the three temperature regimes for ten weeks. Abalone growth was temperature dependent, with growth declining from 4.33 g.month-I for abalone cultured at 18°C to 0.77 g.month-I at 24°C. Dietary protein could be reduced from 26 to 18 % provided dietary energy levels were maintained at 13.5 MJ.kg- l • A dietary energy level of 11.6 MJ.kg-1 was insufficient to meet the energetic requirements of H midae regardless of the protein content of the diet. The effects of water temperature and body size on the protein requirements of H midae were investigated by culturing abalone at temperatures within the optimal range for abalone farming (i.e. 14, 16 and 18°C). Three size classes of abalone (15, 50 and 80 mm) were fed formulated feed containing graded levels of dietary protein (20, 26, 32, 38 and 44 %) under controlled laboratory conditions for 12 weeks, and, in a separate experiment, under commercial farm conditions for 24 weeks. It was not possible to convincingly define the optimal protein levels for abalone of different sizes in this experiment because growth rates fell below average commercial growth rates obtained on farms. Growth was temperature dependent in the laboratory trial, with the rate of weight gain of the 15 mm (ANOV A: p=0.002) and 50 mm abalone (ANOV A: p=0.02) increasing significantly with an increase in temperature from 14 to 18°C. In the farm trial, dietary protein content did not affect the growth rate of the 10-15 or 80 mm abalone (ANOVA: p>0.05), however, the 50 mm abalone displayed significantly higher weight gain on the 32 % (4.72±0.20 g.month-I ) and 38 % (5.01±0.34 g.month-I ) protein diets compared to those fed the 20 % protein diet (3.75±0.13 g.month-I ) (ANOVA: p=O.OI). Although definition of optimal dietary protein levels were not possible, the effects of dietary protein content and water temperature on the growth of H midae were independent signifying that the protein requirements of abalone are temperature independent. In addition, there was no evidence to indicate that abalone of the different sizes tested here had different dietary protein requirements. The size specific dietary lipid and protein requirements of H midae were investigated by feeding two size classes of abalone (30 and 60 mm initial shell length) diets containing graded levels of dietary lipid (4, 7, 10, 13 and 16 %) and protein (34 - 39 %) for 12 weeks. The 30 and 60 mm abalone were stocked at 7 (n=200) and 9 % (n=36) of the available basket surface area respectively and each diet was fed to four baskets of abalone of each size class. The protein requirements of H. midae are influenced by the amount of available dietary energy and thus it is possible that the ability of abalone to utilise lipids as a source of energy differs in the presence of varying levels of dietary protein. High levels of dietary lipid negatively affected the growth, condition factor and soft tissue glycogen content of both size classes of abalone. This negative effect was greater in the 30 mm size class compared to the 60 mm abalone. The corresponding increase in feed consumption and feed conversion ratio in response to increasing levels of dietary protein also provides evidence that abalone are unable to utilise dietary lipids as an energy source and high levels of dietary lipid probably inhibit the uptake of carbohydrates and protein. High dietary lipid levels did however appear to promote gonad maturation. It was possible to reduce dietary protein from 34 to 20 % without negatively affecting growth through the maintenance of dietary energy levels and thus it is recommended that future experiments on the energy content of formulated feeds should focus on the improved use of carbohydrates. Reductions in the protein portion of formulated feeds for H. midae are possible provided the diet contains sufficient levels of energy supplied from carbohydrates. As the ability of abalone to utilise dietary lipid is limited, lipids are unlikely to play a significant role as an energy source in abalone feeds. Further investigations should focus on the utilisation of various carbohydrate sources in abalone feeds.]]> Thu 13 May 2021 06:48:07 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:4360 Thu 13 May 2021 06:16:05 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:9677 Thu 13 May 2021 06:15:31 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:3965 1 mM resulted in the precipitation of protein-nanoparticle bioconjugates, due to unfavourable acidic conditions. This demonstrated that the nanoparticles were binding to and becoming stabilised by general protein in the cell-free solution. Upon addition of a sodium-bicarbonate buffer, a general increase in Pt(IV) reduction to Pt(II) was observed. The addition of the buffer also resulted in an unexplained change in particle morphology and for this reason was not used in subsequent investigations. Polyvinylpyrrolidone (PVP) was shown to compromise the reduction rate of the Pt(IV) ion by SRB cells. The presence of extracellular NP’s was suggested by the colour of the supernatant turning brown and the A334 increasing over time. Attempts to visualise the particles by transmission electron microscopy (TEM) resulted in an unexpected phenomenon where nanoparticles could be observed to form dynamically upon irradiation by the electron beam. Extended irradiation by the electron beam also resulted in structural changes of the particles occurring during observation. An increase in temperature was shown to increase the reduction rate which in turn resulted in particles decreasing in size. The starting pH was shown to have a significant effect on the reduction rate and particle morphology although specific trends could not be identified. In conclusion, the cell-soluble extract from the sulfate-reducing consortium investigated, is capable of Pt(0) nanoparticle synthesis. Precise control over the particle morphology was not attained although the mechanism was further clarified and optimal conditions for nanoparticle synthesis were determined.]]> Thu 13 May 2021 06:12:22 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:9692 Thu 13 May 2021 06:07:54 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:10388 Thu 13 May 2021 05:44:33 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:4838 Thu 13 May 2021 05:38:18 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:5299 Thu 13 May 2021 05:26:18 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:5110 Thu 13 May 2021 05:20:31 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:10652 Thu 13 May 2021 05:19:37 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:10387 Thu 13 May 2021 05:19:10 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:5113 Thu 13 May 2021 05:16:26 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:5247 Thu 13 May 2021 05:14:49 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:4211 Thu 13 May 2021 04:48:45 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:32275 Thu 13 May 2021 04:48:23 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:4370 Thu 13 May 2021 04:48:05 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:10429 Thu 13 May 2021 04:41:37 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:4836 Thu 13 May 2021 04:39:58 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:10392 Thu 13 May 2021 04:16:14 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:10600 Thu 13 May 2021 04:02:03 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:10393 Thu 13 May 2021 03:56:55 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:3993 Thu 13 May 2021 03:50:16 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:4764 Thu 13 May 2021 03:43:02 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:5791 0.05). Total integrated mesozooplankton abundance and biomass during the study ranged between 3934.9 and 308521.4 ind.m-2 (mean = 47198.19; SD±62411.4 ind.m-2) and between 239.8 and 4614.3 mg Dwt.m-2 (mean = 1338.58; SD ±1060.5), respectively. Again, there were no significant spatial patterns in the total mesozooplankton abundance or biomass within the region of study (p>0.05). No significant correlations were found between biological (chlorophyll-a concentrations and zooplankton abundance) and physico-chemical variables (temperature and salinity) (p>0.05). The total mesozooplankton community was numerically dominated by copepods of the genera Pleuromamma, Calanus, Oncaea and Oithona. Other important representatives of the mesozooplankton community included the tunicate, Salpa thompsoni, and the pteropod, Limacina retroversa. At the 40% similarity level, numerical analysis identified five distinct mesozooplankton groupings within the survey area. Differences between the groupings were associated with changes in the relative contribution of numerically dominant species rather than the presence or absence of individual species. No groupings were associated with any specific feature of the front within the survey area. The feeding rates of the six most numerically abundant mesozooplankton species (Calanus simillimus, Limacina retroversa, Pleuromamma abdominalis, Clausocalanus breviceps, Oncaea conifera, Salpa thompsoni) accounting for on average 39% of the total mesozooplankton counts, were investigated using the gut fluorescence technique. For all species, the total gut pigment contents during the night time were significantly higher than the daytime values (p<0.05 for all species). The gut evacuation rates (k) for selected mesozooplankton ranged between 0.14 and 0.81 h-1. The ingestion rates ranged between 147.8 and 5495.4 ng(pigm)ind-1.day-1 which corresponded to a daily ration of between 2.4 and 10.9% body carbon. The combined grazing impact of the selected species on the daily phytoplankton standing stock was highly variable and ranged between 1.2 and 174.1% with an average of 27.3% (SD±38.78%) within the survey area. The highest grazing impact (>60%) was typically associated with those stations where the pteropod, L. retroversa, and the tunicate, S. thompsoni, contributed more than 5% of the total mesozooplankton counts. No significant differences were found in the grazing impact of any or all selected species situated either north, south or in the immediate vicinity of the front (p>0.05 in all cases). The lack of defined spatial patterns in the mesozooplankton abundance and community structure suggests that the STC did not act as a significant biogeographic barrier to the distribution of mesozooplankton during the study. It is presumed that the large scale mixing event caused by a storm prior to this study was responsible for the observed lack of elevated biological activity within the region of the STC.]]> Thu 13 May 2021 03:30:47 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:5566 Thu 13 May 2021 03:16:21 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:10615 Thu 13 May 2021 02:57:03 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:10468 Thu 13 May 2021 02:54:20 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:10719 Thu 13 May 2021 02:48:24 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:10658 Thu 13 May 2021 02:25:28 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:4597 Thu 13 May 2021 01:56:55 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:10539 Thu 13 May 2021 01:51:45 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:10704 Thu 13 May 2021 01:34:32 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:5469 Thu 13 May 2021 01:31:54 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:10320 Thu 13 May 2021 00:57:54 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:4590 Thu 13 May 2021 00:49:42 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:5468 Thu 13 May 2021 00:24:49 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:4064 Thu 13 May 2021 00:22:11 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:10315 Thu 13 May 2021 00:16:57 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:5495 Thu 13 May 2021 00:13:28 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:10616 Thu 13 May 2021 00:05:46 SAST ]]> https://vital.seals.ac.za/vital/access/manager/Repository/vital:3978 Fri 06 Aug 2021 11:41:17 SAST ]]>