${session.getAttribute("locale")} 5 A sociological analysis of the sex education of young adult white womxn and their understandings and practices of ‘Safe Sex’ Wed 27 May 2020 16:29:16 SAST ]]> An investigation into the use of visualisation processes as a teaching strategy to enhance number sense Wed 27 May 2020 16:09:48 SAST ]]> A critical appraisal of Saule’s portrayal of women as Amadelakufa (resilient) Wed 27 May 2020 12:58:55 SAST ]]> An ideological analysis of the construction of the young female action hero as feminist hero in The Hunger Games film franchise Wed 22 Apr 2020 12:16:33 SAST ]]> A morphogenetic study of ESD inclusion in Namibia’s Senior Primary English curriculum: a case study of the Khomas Region Wed 22 Apr 2020 10:26:10 SAST ]]> A computational analysis to decipher the pathways of stability, uncoating and antigenicity of human enterovirus capsids Wed 21 Aug 2019 14:59:16 SAST ]]> Application of quality by design to the manufacture of a multiparticulate prednisone dosage form Wed 18 Sep 2019 11:36:12 SAST ]]> VIVA Comrade Moss Wed 17 Jul 2019 10:02:07 SAST ]]> A dynamics based analysis of allosteric modulation in heat shock proteins Wed 15 Apr 2020 14:10:58 SAST ]]> Choreographies of protest performance as recruitment to activism and the movement of perception during the 2015 re-emergence of student activism at Rhodes University Wed 15 Apr 2020 14:02:40 SAST ]]> An exploration of the overlap between open source threat intelligence and active internet background radiation Wed 15 Apr 2020 13:20:46 SAST ]]> Come listen quickly Wed 15 Apr 2020 10:53:39 SAST ]]> A critical investigation of leadership in a Technical, Vocational Education and Training college in the Eastern Cape Wed 15 Apr 2020 09:43:45 SAST ]]> An investigation into the knowledge a Grade one teacher uses to develop the number sense of learners with mathematics learning difficulties Wed 15 Apr 2020 09:43:39 SAST ]]> Biodiversity conservation and rural livelihoods across four nature reserves in the Eastern Cape Province, South Africa: Striving towards a balance between livelihoods and conservation Wed 11 Mar 2020 10:32:58 SAST ]]> Between nationalism and transnationalism: entanglements of history, individual narrative, and memory in diaspora spaces in selected transnational fiction Wed 08 Apr 2020 14:25:24 SAST ]]> A formative intervention study of how learner voice and leadership can be developed within a Learner Representative Council (LRC) in an urban combined school, Namibia Wed 06 May 2020 17:04:59 SAST ]]> An exploratory study on UNAM’s opportunities of being an entrepreneurial university Wed 06 May 2020 16:16:00 SAST ]]> The design of a modal interchange in Korsten, Port Elizabeth: santuary for the urban nomad Wed 04 Dec 2019 11:22:04 SAST ]]> A historical sociolinguistic study on the conceptualisation and application of justice and law kwisizwe samaXhosa as documented in and extracted From SEK Mqhayi And W.W. Gqoba’s selected writings Wed 01 Apr 2020 15:46:51 SAST ]]> A mixed methods investigation of students’ attitudes towards statistics and quantitative research methods: a focus on postgraduate psychology students at a South African university Wed 01 Apr 2020 12:31:38 SAST ]]> An assessment of temporal changes in selective biological characteristics of chokka squid (Loligo reynaudii d’Órbigny, 1845) 0.05) results when temperature was analysed by t-test between 2002 and 2015. There was also a weak correlation between length frequency and the total squid catch in a given year (F Statistic (df = 1; 13) is 3.686 and 5.394 for males and females respectively, R² is 0.221 for males and 0.293 for females), but too weak to interpret, given the lack of other supporting data and the short time series. The ML_TW relationship showed no significant trends between the years for either sex. There was also no correlation between the ML_TW and total squid catch or temperature. A white caecum occurred significantly more often in males than in females (dof = 1; p < 0.05) from General Linear Model (GLM), indicating that the presence of non-feeding males in the spawning grounds may be linked to the behaviour of spawning squid.]]> Tue 28 Apr 2020 15:20:19 SAST ]]> Cyclooxygenase-1 as an anti-stroke target: potential inhibitor identification and non-synonymous single nucleotide polymorphism analysis Tue 28 Apr 2020 12:18:22 SAST ]]> BEd foundation phase fourth year student teachers’ self-efficacy beliefs towards teaching mathematics and the self-reported factors that influence these self-efficacy beliefs Tue 26 May 2020 13:56:29 SAST ]]> An investigation of the experiences of psychotherapists regarding Ubuntu in their psychotherapy practice: an interpretative phenomenological analysis Tue 26 May 2020 11:01:28 SAST ]]> A study of why some physic concepts in the South African Physical Science curriculum are poorly understood in order to develop a targeted action-research intervention for Newton’s second law Tue 26 May 2020 10:12:28 SAST ]]> A novel, improved throughput bioassay for determining the delative speed of antimalarial drug action using fluorescent vitality probes Tue 24 Mar 2020 14:58:57 SAST ]]> Building a flexible and inexpensive multi-layer switch for software-defined networks Tue 21 Apr 2020 16:08:48 SAST ]]> An investigation of the internal challenges that hinder sustainability of the Furntech Nyanga incubates Tue 21 Apr 2020 15:54:47 SAST ]]> A reappraisal of the origin of the Hotazel Fe-Mn Formation in an evolving early Earth system through the application of mineral-specific geochemistry, speciation techniques and stable isotope systematics Tue 19 May 2020 12:03:46 SAST ]]> A 150 MHz all sky survey with the Precision Array to Probe the Epoch of Reionization Tue 17 Mar 2020 16:29:38 SAST ]]> A critique of the language of record in South African courts in relation to selected university language policies Tue 14 Apr 2020 13:28:06 SAST ]]> Carnivore intra-guild competition in Selati Game Reserve, Limpopo Province, South Africa Tue 12 May 2020 14:52:38 SAST ]]> An intervention on supporting teachers’ understanding of and mediation of learning of stoichiometry in selected schools in the Zambezi Region Tue 12 May 2020 14:45:38 SAST ]]> An evaluation of social sustainability practices in an agri-based private company in the Eastern Cape Tue 12 May 2020 13:00:39 SAST ]]> Afromelampsalta, a new genus, a new species, and five new combinations of African cicadettine cicadas (Hemiptera: Cicadidae: Cicadettinae): Tue 12 May 2020 12:39:33 SAST ]]> A personality-based surveillance model for Facebook apps Tue 12 May 2020 10:42:59 SAST ]]> An investigation of how selected Namibian grade 9 geography textbooks are reorienting school geography to education for sustainable development Tue 12 May 2020 09:44:38 SAST ]]> Carbon isotope evidence for water-column carbon and iron cycling in the Paleoproterozoic ocean and implications for the early biological pump: supplementary data file Tue 10 Mar 2020 16:45:56 SAST ]]> Biogeographic patterns of endolithic cyanobacteria and their negative impacts on mussels along the South African coast Tue 05 May 2020 14:31:25 SAST ]]> An investigation into the emergence of teacher leadership: a case study at an urban state senior secondary school in the Oshana Region, Namibia Tue 05 May 2020 13:21:25 SAST ]]> A systematic review of the efficacy of internet-based interventions for depression and anxiety disorders: the possibilities and limitations for feasibility within South Africa Tue 05 May 2020 13:18:27 SAST ]]> Combining DNA barcoding and morphology to identify larval fishes from the nearshore environment off the south-east coast of South Africa Tue 05 May 2020 12:46:24 SAST ]]> An ideological analysis of the construction of masculinity in the South African superhero comic book, Kwezi Tue 05 May 2020 12:11:24 SAST ]]> A comparative analysis of social media brand image of insurance companies in South Africa: a longitudinal study Thu 30 Apr 2020 16:01:43 SAST ]]> An Analysis of Self-Help Agricultural Projects in Rothe Village, Lesotho Thu 30 Apr 2020 15:33:44 SAST ]]> Commonplaces Thu 30 Apr 2020 12:10:44 SAST ]]> An analysis of the availability of and access to credit from the formal financial sector and the performance of SMEs Thu 30 Apr 2020 11:28:43 SAST ]]> Erythrina caffra - kaffirboom Thu 26 Sep 2019 12:09:50 SAST ]]> BODIPY dyes for application in the photo-oxidation of pollutants, photodynamic antimicrobial chemotherapy, and nonlinear optics Thu 26 Mar 2020 10:26:20 SAST ]]> A critical analysis of NGOs in addressing HIV and AIDS in the context of gendered inequality: The case of Makhanda, Eastern Cape, South Africa Thu 23 Apr 2020 14:59:38 SAST ]]> An analysis of how visualisation capabilities in dynamic geometric software develop meaning-making of mathematical concepts in selected Grade 11 learners Thu 23 Apr 2020 14:03:34 SAST ]]> Anticholinesterase and Antioxidant Activities of Spilanthes filicaulis Whole Plant Extracts for the Management of Alzheimer’s Disease Thu 19 Mar 2020 09:21:24 SAST ]]> A content analysis of psychology Masters theses from South African universities Thu 15 Aug 2019 12:31:50 SAST ]]> An investigation into the nature and adequacy of tax compliance tools available to assist small businesses in the state of Oklahoma in the United States of America Thu 09 Apr 2020 11:22:11 SAST ]]> An exploration of how Grade 3 Foundation Phase teachers develop basic scientific process skills using an inquiry-based approach in their classrooms Thu 07 May 2020 16:08:59 SAST ]]> Crossing shades Thu 07 May 2020 14:02:58 SAST ]]> An assessment of the effectiveness of the Crossways Farm Village constructed wetland in the treatment of domestic wastewater Thu 07 May 2020 13:06:55 SAST ]]> A tribute to you Thu 07 May 2020 12:45:56 SAST ]]> An Analysis of Internet Background Radiation within an African IPv4 netblock Thu 02 Apr 2020 16:02:03 SAST ]]> A social-ecological investigation into urban domestic gardens and avifauna in medium-sized towns of the Eastern Cape, South Africa Sun 10 May 2020 12:10:34 SAST ]]> A comparative analysis with selected jurisdictions of structural challenges facing the South African office of the tax ombud Sat 28 Mar 2020 16:23:01 SAST ]]> Careerism and capitalism as women’s wmancipation: a critical analysis of Rand Merchant Bank’s ‘Athena Programme,’ South Africa Sat 28 Mar 2020 15:20:00 SAST ]]> A critical analysis of the de-peasantisation process in Nepal with specific reference to the role of state land policies since the 1950s Sat 28 Mar 2020 13:21:01 SAST ]]> An investigation of learning and emerging knowledge in the Mpophomeni Sanitation Education Project, Howick, KwaZulu-Natal Sat 14 Mar 2020 12:17:44 SAST ]]> A critique of translated (isiXhosa to English) sworn statements by the South African Police Service Mon 30 Mar 2020 16:01:51 SAST ]]> Careerism and capitalism as women’s emancipation: a critical analysis of Rand Merchant Bank’s ‘Athena Programme,’ South Africa Mon 26 Aug 2019 12:24:49 SAST ]]> Analysing emergent time within an isolated Universe through the application of interactions in the conditional probability approach Mon 25 May 2020 11:29:28 SAST ]]> An investigation into the readiness of open source software to build a Telco Cloud for virtualising network functions Mon 21 Oct 2019 12:39:00 SAST ]]> A classification of large wetlands in Africa’s elevated drylands based on their formation, structure, and hydrological functioning using Earth Observation (EO) data and Geographic Information System (GIS) Mon 20 Apr 2020 16:22:47 SAST ]]> A sociological understanding of contemporary child marriage in Mabvuku, Harare, Zimbabwe Mon 20 Apr 2020 14:35:20 SAST ]]> Computer aided approaches against Human African Trypanosomiasis 3000 cases and controls) are required. Macrophage migration inhibitory factor (MIF) is a cytokine that is important in both innate and adaptive immunity that has been shown to play a role in T. brucei pathogenicity using murine models. A total of 27 missense SNVs were modelled using homology modelling to create MIF protein mutants that were investigated using in silico effect prediction tools, molecular dynamics (MD), Principal Component Analysis (PCA), and Dynamic Residue Network (DRN) analysis. Our results demonstrate that mutations P2Q, I5M, P16Q, L23F, T24S, T31I, Y37H, H41P, M48V, P44L, G52C, S54R, I65M, I68T, S75F, N106S, and T113S caused significant conformational changes. Further, DRN analysis showed that residues P2, T31, Y37, G52, I65, I68, S75, N106, and T113S are part of a similar local residue interaction network with functional significance. These results show how polymorphisms such as missense SNVs can affect protein conformation, dynamics, and function. Trypanosomes are auxotrophic for folates and pterins but require them for survival. They scavenge them from their hosts. PTR1 is a multifunctional enzyme that is unique to trypanosomatids that reduces both pterins and folates. In the presence of DHFR inhibitors, PTR1 is over-expressed thus providing an escape from the effects of DHFR inhibition. Both TbPTR1 and TbDHFR are pharmacologically and genetically validated drug targets. In this study 5742 compounds were screened using molecular docking, and 13 promising binding modes were further analysed using MD simulations. The trajectories were analysed using RMSD, Rg, RMSF, PCA, Essential Dynamics Analysis (EDA), Molecular Mechanics Poisson–Boltzmann surface area (MM-PBSA) binding free energy calculations, and DRN analysis. The computational screening approach allowed us to identify five of the compounds, named RUBi004, RUBi007, RUBi014, RUBi016 and RUBi018 that exhibited antitrypanosomal growth activities against trypanosomes in culture with IC50 values of 12.5 ± 4.8 μM, 32.4 ± 4.2 μM, 5.9 ± 1.4 μM, 28.2 ± 3.3 μM, and 9.7 ± 2.1 μM, respectively. Further when used in combination with WR99210 a known TbDHFR inhibitor RUBi004, RUBi007, RUBi014 and RUBi018 showed antagonism while RUBi016 showed an additive effect. These results indicate that the four compounds might be competing with TbDHFR while RUBi016 might be more specific for TbPTR1. These compounds provide scaffolds that can be further optimised to improve their potency and specificity. Lastly, using a systematic approach we derived CHARMM force-field parameters to accurately describe the TbrPDEB1 bi-metal catalytic center. For dynamics, we employed mixed bonded and non-bonded approach. We optimised the structure using a two-layer QM/MM ONIOM (B3LYP/6-31(g): UFF). The TbrPDEB1 bi-metallic center bonds, angles, and dihedrals were parameterized by fitting the energy profiles from Potential Energy Surface (PES) scans to the CHARMM potential energy function. The parameters were validated by means of MD simulations and analysed using RMSD, Rg, RMSF, hydrogen bonding, bond/angle/dihedral evaluations, EDA, PCA, and DRN analysis. The force-field parameters were able to accurately reproduce the geometry and dynamics of the TbrPDEB1 bi-metal catalytic center during MD simulations. Molecular docking was used to identify 6 potential hits, that inhibited trypanosome growth in vitro. The derived force-field parameters were used to simulate the 6 protein-ligand complexes with the aim of elucidating crucial protein-ligand residue interactions. Using the most potent ligand RUBi022 that had an IC50 of 14.96 μM we were able to identify key residue interactions that can be of use in in silico prediction of potential TbrPDEB1 inhibitors. Overall we demonstrate how bioinformatics tools can complement current disease eradication strategies. Future work will focus on identifying variants identified in Genome Wide Association Studies and partnering with wet labs to carry out further enzyme-ligand activity relationship studies, structure determination or characterisation of appropriate protein-ligand complexes by crystallography, and site specific mutation studies]]> Mon 20 Apr 2020 14:09:43 SAST ]]> A comparison of representations of the imperative of higher education change as „transformation‟ versus „decolonisation‟ in South African public discourse Mon 20 Apr 2020 12:56:08 SAST ]]> An exploration of organizational expectations of different generations of employees during change Mon 20 Apr 2020 11:21:42 SAST ]]> Chinatown Mon 20 Apr 2020 11:00:42 SAST ]]> A five year comparative analysis of annual baseline neurocognitive test scores for South African high school athletes Mon 18 May 2020 11:22:15 SAST ]]> Decent work and informal employment: the case of Bulawayo Metropolitan Province (Central Business District) Zimbabwe Mon 16 Mar 2020 16:30:06 SAST ]]> A combined modelling approach for simulating channel–wetland exchanges in large African river basins Mon 14 Oct 2019 12:24:57 SAST ]]> An analysis of the public reporting of organisational resilience found within the integrated reports of a large state-owned enterprise Mon 13 Apr 2020 16:15:37 SAST ]]> An analysis of indicators disclosed in the integrated annual reports of selected South African retailers Mon 13 Apr 2020 14:51:38 SAST ]]> An investigation into the tax consequences for individuals performing work abroad Mon 13 Apr 2020 12:10:37 SAST ]]> An explorative study of Makana local football association coaches characteristics, experiences and needs Mon 13 Apr 2020 11:35:38 SAST ]]> An investigation of the security of passwords derived from African languages Mon 09 Sep 2019 11:49:44 SAST ]]> An investigation of the influence of knowledge-production and learning processes on complex practices in a community-driven citizen science initiative: A nature conservation case study Mon 09 Mar 2020 14:47:28 SAST ]]> A q-metholological approach to audience reception of public awareness messages on sexual violence Mon 06 Apr 2020 15:27:23 SAST ]]> Age of squid Loligo reynaudii d’Orbigny, 1845, and its possible use to test effectiveness of the closed season in protecting this resource Mon 04 May 2020 12:25:13 SAST ]]> A case study of how a leader shaped the culture of an organisation during a post-merger integration Fri 22 May 2020 15:13:13 SAST ]]> An investigation into the readiness of open source software to build a telco cloud for virtualizing network functions Fri 20 Mar 2020 15:48:39 SAST ]]> Assessment of the microbial quality of various domestic rainwater harvesting systems and the suitability of a nano based treatment method Fri 19 Jul 2019 15:12:52 SAST ]]> An analysis of purple truths: an alternative history of the school of journalism and media studies at Rhodes University. Exploring the possibilities of digital media for telling history through multiple voices Fri 17 Apr 2020 16:57:40 SAST ]]> Application of machine learning, molecular modelling and structural data mining against antiretroviral drug resistance in HIV-1 Fri 17 Apr 2020 16:36:39 SAST ]]> Activity of diverse chalcones against several targets: statistical analysis of a high-throughput virtual screen of a custom chalcone library Fri 17 Apr 2020 11:28:41 SAST ]]> Chasing Eden: Margaret Atwood’s MaddAddam trilogy and the value of reading in a technological age Fri 15 May 2020 15:00:52 SAST ]]> A Bayesian approach to tilted-ring modelling of galaxies Fri 15 May 2020 14:53:52 SAST ]]> An initial investigation into biological control options for Schinus terebinthifolia in South Africa Fri 14 Jun 2019 10:18:54 SAST ]]> An exploration of formative assessment in primary school Natural Sciences classrooms Fri 08 May 2020 16:58:20 SAST ]]> A comparative study of conceptualisations and practices of inclusion as an aspect of social justice in three teacher education institutions in Canada, South Africa and Zimbabwe Fri 08 May 2020 11:08:18 SAST ]]> A theory-based approach to evaluating a Continuing Teacher Professional Development Programme aimed at strengthening environment and sustainability education Fri 08 May 2020 10:54:18 SAST ]]> An investigation into the fire regimes of the upper Tsitsa River catchment Fri 08 May 2020 10:17:15 SAST ]]> 300 grams Fri 03 Apr 2020 18:07:53 SAST ]]>